Automated Analysis of Microscopic Images of Isolated Pancreatic Islets
نویسندگان
چکیده
منابع مشابه
Isolated Pancreatic Islets
It is the aim of this study to characterize and quantify the cells within isolated rat islets that express MHC class 11 antigens. A set of five monoelonal antibodies and two polyclonal antisera of defined specificlty were used in combination with a newly devised procedure for three·dimensional immunofluorescence evaluation of intact islets. It is shown that in addition to passen· ger cells, suc...
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Inosine is a potent primary stimulus of insulin secretion from isolated mouse islets. The inosine-induced insulin secretion was totally depressed during starvation, but was completely restored by the addition of 5mmi-caffeine to the medium and partially restored by the addition of 5mM-glucose. Mannoheptulose (3mg/ml) potentiated the eflect of lOmM-inosine in islets from fed mice. The mechanism ...
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Objective: Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level.This study was undertaken to investigateits direct effect on the islets of Langerhans. Material and Methods: Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer containi...
متن کاملImmunoelectron microscopic localization of MHC structures in isolated pancreatic rat islets.
An immunogold-silver enhancement technique, which combines effective labeling of viable isolated islets with the ultrastructural resolution of cytological details, was applied in electron microscopy to identify major histocompatibility complex (MHC) structures on islet cells. Incubation of freshly isolated islets from CAP (RT1c) and LEW (RT1l) rats with OX18, an MHC class I antibody, showed str...
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Numerous electron microscopic studies have been published on the pancreatic islets in a variety of animal species, but those on the normal human islets are relatively few (YOKOH, IIZUKA and KANO, 1959; EKHOLM and EDLUND, 1959; GUSEK and KRACHT, 1959, 1961; ZAGURY, BRUX, ANCLA and LEGER, 1961; LACY, 1961, 1964; LAZARUS and YOLK, 1962; BENCOSME, ALLEN and LATTA, 1963; GREIDER, ELLIOTT and ZOLLING...
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ژورنال
عنوان ژورنال: Cell Transplantation
سال: 2016
ISSN: 0963-6897,1555-3892
DOI: 10.3727/096368916x692005